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Life Sciences
New findings from China Agricultural University in the area of life sciences described
June 18th, 2008
According to recent research published in the journal Nature Protocols, "Rapid advances in the fields of DNA vaccines and gene therapy have produced increased demands for large quantities of recombinant plasmid DNA. The protocol presented here extracts plasmid DNA in a scalable continuous process based on an alkaline lysis protocol." "In the process, harvested bacteria are passed through two mixing chambers at controlled speeds to effect lysis and control alkalinity. The resulting solution is passed through a series of filters to remove contaminants and then ethanol precipitated. This process replaces all the centrifugation steps before obtaining crude plasmid...
Source: Vaccine Weekly (2008-06-18)
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