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Hepatitis B Virus
Research on hepatitis B virus detailed by G.S. Kee and co-authors
July 30th, 2008
According to a study from London, the United Kingdom, "Virus-like particles (VLPs) are expressed intracellularly in Saccharomyces cerevisiae and the recovery process involves the use of a detergent, which facilitates the release of VLP from host cell components. The detergent-mediated liberation of VLPs is a critical step in primary recovery and is responsible for setting the backdrop for subsequent purification in terms of product yield and characteristics of the process stream." "In this paper the use of Triton X-100 detergent for the recovery of lipid envelope VLPs, using the hepatitis B surface antigen (HBsAg) as the VLP model, was investigated. To develop a...
Source: Vaccine Weekly (2008-07-30)
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