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Life Sciences
Studies in the area of life sciences reported from H.Y. Chen and co-researchers
August 6th, 2008
According to recent research published in the journal Veterinary Immunology and Immunopathology, "The encoding sequence for duck IL-18 was obtained, using reverse transcription-polymerase chain reaction, from mRNA harvested from Con A-stimulated Gushi (GS) duck splenic mononuclear cells. Recombinant duck IL-18 (rduIL-18) was produced in a prokaryotic expression system." "In vitro bioactivity of rduIL-18 was determined in a lymphocyte proliferation assay and in vivo bioactivity of rduIL-18 was assessed by addition to a vaccine. Monoclonal antibody (mAb) and polyclonal antibodies (pAbs) specific for rduIL-18 were generated and subsequently characterized by ELISA,...
Source: Vaccine Weekly (2008-08-06)
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