Vaccine Weekly
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Protein Encoding
Reports summarize protein encoding research from C.Y. Wang and co-authors
August 27th, 2008
"Due to appearance of new genotypes of Newcastle disease virus (NDV) with no cross-protection and with vaccine strains, some outbreaks have been reported in Taiwan that caused significant damage to the poultry industry. A reliable assay protocol, (RAPID) -bioactive amplification with probing (BAP), for detection of NDV that uses a nested PCR and magnetic bead-based probe to increase sensitivity and specificity, was developed," scientists in Neipu, Taiwan report. "Primers and probes were designed based on the conserved region of the F protein-encoding gene sequences of all NDV Taiwan isolates. The optimal annealing temperature for nested reverse transcription-polymerase...
Source: Vaccine Weekly (2008-08-27)
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