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Gastroenteritis
New gastroenteritis study findings recently were reported by investigators at University of Tampere, Vaccine Research Center
December 10th, 2008
New research, 'One-step quantitative RT-PCR for the detection of rotavirus in acute gastroenteritis,' is the subject of a report. According to a study from Tampere, Finland, "The standard diagnosis of rotavirus gastroenteritis is based on the demonstration of rotavirus antigen in stools using an enzyme immunoassay (EIA). In this study, a one-step quantitative RT-PCR (Q-PCR) was used for sensitive detection of rotavirus in diarrheal stools." "The primers and TaqMan probe for the Q-PCR were selected from a highly conserved region of the non-structural protein 3 (NSP3) of rotavirus. After validation, the test was applied to study rotavirus EIA positive (N=25) and EIA...
Source: Vaccine Weekly (2008-12-10)
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