Gene Therapy
Studies from H.T. Li et al in the area of gene therapy described
Published in Vaccine Weekly, February 11th, 2009
"In this report, a replication-defective human adenovirus type 3 was constructed by deletion of the whole El gene. A HEp-2 cell-based cell line designated HEp-2/E1 was generated and was shown to complement the function of El deleted mutant. A partial genome (9.5-100 mu) of HAdV-3 was cloned into pPolyll vector to be a backbone plasmid. A shuttle vector carrying eGFP gene was also constructed. The backbone and shuttle plasmids were co-transfected into the HEp-2/E1 cell line...
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Source: Vaccine Weekly (2009-02-11)
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