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Escherichia coli
Data from Wuhan University provide new insights into Escherichia coli
March 18th, 2009
According to a study from Wuhan, People's Republic of China, "PA (protective antigen) is a major immunogen of the vaccine against anthrax. In the present study, a new expression system, Escherichia coli strain Rosetta(TM) 2(DE3), was used for high-level expression of rPA (recombinant PA) whose gene contains 66 rare E. coli codons (9.0% of 733 total PA gene codons)." "The rPA-formed inclusion bodies were washed with Triton X-100 and 2 M urea and solubilized in 5 M urea, followed by a 60%-satd.-ammonium sulfate precipitation. Finally, the untagged rPA was efficiently and rapidly purified by single-step hydrophobic-inter, action chromatography using Phenyl-Sepharose High...
Source: Vaccine Weekly (2009-03-18)
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