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Genetics
Study findings from Mississippi State University broaden understanding of genetics
March 25th, 2009
According to recent research published in the Journal of Virological Methods, "Crossover recombination based on the lambda phage integration/excision functions enables insertion of a gene of interest into a specific locus by a simple one-step in vitro recombination reaction. Recently, a highly efficient recombination system for targeted mutagenesis, which utilizes lambda phage crossover recombination cloning, has been described for a human herpesvirus 2 bacterial artificial chromosome (BAC)." "The disadvantages of the system are that it allows only neutral selection (loss of green fluorescent protein) of desired recombinants and that it regenerates herpesvirus...
Source: Vaccine Weekly (2009-03-25)
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