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Vaccinia Virus
Research from S. Popov and co-researchers in the area of vaccinia virus published
May 6th, 2009
According to recent research from the United States, "We developed a rapid method to generate recombinant vaccinia viruses (rVVs) based upon a bicistronic cassette encoding the gene for green fluorescent protein (GFP) and a foreign gene of interest separated by an internal ribosome entry site (IRES). As proof-of-concept, we inserted a mutant env gene of human immunodeficiency virus (HIV) into the cassette, which was cloned into the vaccinia virus (VV) insertion vector pSC59 under the control of the early-late VV synthetic promoter and flanked by disrupted tk gene sequences." "To generate rVVs, 293T cells were inoculated with wild-type (wt) VV, followed by transfection...
Source: Vaccine Weekly (2009-05-06)
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