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Monoclonal Antibodies
Research results from K. Smith and colleagues update understanding of monoclonal antibodies
July 8th, 2009
"We describe herein a protocol for the production of antigen-specific human monoclonal antibodies (hmAbs). Antibody-secreting cells (ASCs) are isolated from whole blood collected 7 d after vaccination and sorted by flow cytometry into single cell plates," scientists writing in the journal Nature Protocols report. "The antibody genes of the ASCs are then amplified by RT-PCR and nested PCR, cloned into expression vectors and transfected into a human cell line. The expressed antibodies can then be purified and assayed for binding and neutralization. This method uses established techniques but is novel in their combination and application. This protocol can be...
Source: Vaccine Weekly (2009-07-08)
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