Published in Vaccine Weekly, November 2nd, 2011
"Our objective was to identify the genetic defect resulting in his C3 deficiency and measure his ability to mount an adaptive immune response. Fibroblast cell lines were generated from the patient and parents. DNA was isolated and the C3 gene sequenced. Quantitation of C3 expression was performed by immunoprecipitation of 355-methionine labeled protein. Isotype specific...
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