2012 MAR 28 -- Research findings, "Methylation-dependent DNA restriction in Bacillus anthracis," are discussed in a new report. According to the authors of recent research from Bethesda, Maryland, "Bacillus anthracis, the causative agent of anthrax, is poorly transformed with DNA that is methylated on adenine or cytosine. Here we characterize three genetic loci encoding type IV methylation-dependent restriction enzymes that target DNA containing C5-methylcytosine (m5C)."
"Strains in which these genes were inactivated, either singly or collectively, showed increased transformation by methylated DNA. Additionally, a triple mutant with an ~30-kb genomic...
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