Infectious Disease

Novel immunoassay distinguishes tick-borne from Japanese encephalitis

Published in Vector and Zoonosis Week, April 28th, 2003

A novel immunoassay can improve encephalitis diagnosis.

In a recent study from Japan, "a recombinant plasmid that expresses the tick-borne encephalitis (TBE) virus premembrane (prM) and envelope (E) proteins in mammalian cells was constructed."

The modified proteins "retained antigenic and conformational structures similar to those of native virus proteins," reported K. Yoshii and coauthors at Hokkaido University, and "transfected cells released virus-like particles (VLPs), which were 1.13-1.14 g/mL in density and 20-30 nm in diameter, into the culture medium."

"Recombinant E proteins were used for the development of an...

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Source: Vector and Zoonosis Week (2003-04-28)

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