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Malaria Diagnosis

Single-step PCR assay finds and distinguishes Plasmodium parasites

Published in Vector and Zoonosis Week, April 28th, 2003

A novel single-step polymerase chain reaction (PCR) assay can find and distinguish between malaria parasite species in blood.

"The ability to detect and differentiate between Plasmodium falciparum and P. vivax is of great importance for the routine laboratory diagnosis of malaria, donor-blood screening and epidemiological studies," researchers in Greece noted.

"Most PCR-based methods for the discrimination of these two species require nested protocols or an additional hybridization reaction, leading to high labor costs and long turn-around times," according to E. Patsoula and colleagues at the National School of Public Health.

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