Chlamydia
Uracil-DNA glycosylase of Chlamydia pneumoniae characterized
Published in Women's Health Weekly, June 17th, 2004
According to a study from China, "A uracil-DNA glycosylase gene was cloned from Chlamydia pneumoniae AR39 and expressed in E. coli strains BL21 (DE3) and BL21 (DE3) pLysS. After purification by Ni-NTA His Bind Resin and DEAE Sepharose Fast Flow column chromatography, recombinant CpUDG with a specific activity of 1,000,000 U/mg was obtained. The enzymatic activity of the purified CpUDG protein was further characterized using oligodeoxyribonucleotides carrying uracil bases as substrates."
"The base opposite to uracil in double strand DNAs affected uracil...
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