Women's Health Weekly
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Breast Cancer
RT-PCR detects disseminated epithelial cells in BM of breast cancer patients
January 27th, 2005
A real-time RT-PCR assay detects disseminated epithelial cells in the bone marrow of breast cancer patients "Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) is a technique with the potential of improving the quantification of disseminated epithelial cells (DEC) in hematological tissues due to its exquisite sensitivity. This sensitivity may lead to false positivity. Immunocytochemistry (ICC) is regarded as the standard methodology to diagnose DEC," scientists in Belgium report. "In this study," said I.H. Benoy and coworkers, "detection with ICC was compared with quantitative real-time RT-PCR for CK-19 and mammaglobin (hMAM) mRNA in...
Source: Women's Health Weekly (2005-01-27)
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