Women's Health Weekly
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Proteomics
Reports outline proteomics study results from V. Kulasingam et al
March 13th, 2008
According to recent research from Toronto, Canada, "A ''bottom-up'' proteomics approach and a two-dimensional ( strong cation exchange followed by reversedphase) LC-MS/MS strategy on a linear ion trap (LTQ) were utilized to identify and compare expressions of extracellular and membrane-bound proteins in the conditioned media of three breast cell lines (MCF-10A, BT474, and MDA-MB-468). Proteomics analysis of the media identified in excess of 600, 500, and 700 proteins in MCF-10A, BT474, and MDA-MB-468, respectively." "We successfully identified the internal control proteins, kallikreins 5, 6, and 10 ( ranging in concentration from 2 to 50 mu g/liter) in MD-AMB-468...
Source: Women's Health Weekly (2008-03-13)
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