Gene Therapy
Researchers at National Yang-Ming University, Institute of Biochemistry and Molecular Biology target gene therapy
Published in Women's Health Weekly, June 12th, 2008
Research findings, 'Genome-wide mapping and characterization of hypomethylated sites in human tissues and breast cancer cell lines,' are discussed in a new report. "We have developed a method for mapping unmethylated sites in the human genome based on the resistance of TspRI-digested ends to ExoIII nuclease degradation. Digestion with TspRI and methylation-sensitive restriction endonuclease HpaII, followed by ExoIII and single-strand DNA nuclease allowed removal of DNA fragments containing unmethylated HpaII sites," researchers in Taipei, Taiwan report.
"We then used array comparative genomic hybridization (CGH) to map the sequences depleted by these procedures in human...
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