Women's Health Weekly
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Life Sciences
Data on life sciences published by K. Lee and colleagues
July 31st, 2008
According to recent research published in the journal Biochemistry - Moscow, "To achieve high throughput and economical format of 2-D PAGE, comparison between gel size and resolution was conducted on human breast carcinoma cell line (MCF-7/AZ) proteins. SDS gel length showed a weaker influence of separation length on resolution in the second dimension, and there was little benefit of separation distances greater than 15 to 19 cm." "IPG strip separation distances were very important with dramatic increase in resolution of longer gels compared with smaller gels, and maximal resolution was obtained using 18- and 24-cm IPG strips. Loading optimal amount of proteins...
Source: Women's Health Weekly (2008-07-31)
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