Women's Health Weekly
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Breast Cancer
Research in the area of breast cancer reported from Xi'an Jiao Tong University
January 15th, 2009
According to recent research published in the journal Cell Biochemistry and Function, "Membrane and membrane-associated proteins are rich in known or potential pharmaceutical drug targets for carcinogenesis. In order to systemically analyze membrane proteins of human breast cancer, we isolated membrane from MCF-7 cells by sequential extraction by washing with three different buffers, namely, phosphate buffer (5 mM, pH 8.0), Tris (40 mM, pH 9.5), and sodium carbonate (100 mM pH 11)." "The extracted proteins were separated by two-dimensional gel electrophoresis (2-DE) using cup-loading and were then analyzed by peptide mass fingerprinting (PMF). A total of 137...
Source: Women's Health Weekly (2009-01-15)
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