Women's Health Weekly
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Breast Cancer
New breast cancer data have been reported by D. Dietrich and co-authors
June 25th, 2009
According to a study from Berlin, Germany, "A procedure for simultaneous quantification of DNA methylation of several genes in minute amounts of sample material was developed and applied to microdissected formalin-fixed and paraffin-embedded breast tissues. The procedure is comprised of an optimized bisulfite treatment protocol suitable for samples containing only few cells, a multiplex preamplification and subsequent locus specific reamplification, and a novel quantitative bisulfite sequencing method based on the incorporation of a normalization domain into the PCR product." "A real-time PCR assay amplifying repetitive elements was established to quantify low amounts...
Source: Women's Health Weekly (2009-06-25)
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