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Burkholderia Outbreaks

Flagellin P-based PCR detected Burkholderia mallei in equine glanders outbreak

Published in World Disease Weekly, June 27th, 2006

Flagellin P-based PCR detected Burkholderia mallei from an equine glanders outbreak in the United Arab Emirates.

According to recent research from Germany, "A polymerase chain reaction (PCR) assay targeting the flagellin P (fliP)-I S407A genomic region of Burkholderia mallei was developed for the specific detection of this organism in pure cultures and clinical samples from a recent outbreak of equine glanders.

"Primers deduced from the known fliP-IS407A sequence of B. mallei American Type Culture Collection (ATCC) 23344(T) allowed the specific amplification of a 989-bp fragment from each of the 20 B. mallei strains investigated,...

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