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2009 OCT 5 ... demonstrated to enhance and support the scalable manufacturing and growth of human embryonicstem cells (hESCs). Through its ongoing collaboration with Geron, Corning ... of hESCs in a defined (animal-free) medium. Data presented today at the World Stem Cell Summit in Baltimore, Md., will show multi-passage growth of multiple undifferentiated ...
2009 NOV 9 ... "The feasibility of inducing endocrine pancreatic differentiation of embryonicstem (ES) cells has been well documented. However, whether ES cells possess the ... Keywords: People's Republic of China, Guangzhou, Dexamethasone, Drugs, Embryonic Stem Cell Research, Endocrine, Enzyme Research, Gastroenterology, Pancreas, Pharmaceuticals, ... in suspension to form embryoid bodies (EBs). These EBs were cultured in differentiating medium containing varying concentrations of sodium butyrate. The effects of activinA and ...
2009 NOV 2 ... New research, 'Human embryonicstemcell lines derived from single blastomeres of two 4-cell stage embryos,' is the ... development and the 16 blastomeres were individually cultured in sequential medium. On Day 3 or 4, the blastomere-derived embryos were plated on inactivated mouse ...
2009 NOV 23 ... Academy of Sciences of the United States of America report. "Human embryonicstem cells (hESCs) were differentiated to cardiomyocytes by using activin A and BMP-4 ... comprising only cardiomyocytes. Implantation of these patches resulted in 10-fold larger cell grafts compared with patches composed only of cardiomyocytes. Moreover, the preformed ... orbital shaker to create human cardiac tissue patches. Optimization of patch culture medium significantly increased cardiomyocyte viability in patch centers. These patches, ...
2009 NOV 2 ... hepatocyte growth factor (HGF) and G5 supplement, was used to induce rhesus monkey embryonicstem cells (rESC) directly into neuroepithelial (NE) cells. Under these conditions, the ... of NE cells did not require the formation of embryoid bodies or co-culture with other cell types," scientists in Beijing, People's Republic of China report. "The ... "A simple monoculture system. combined with a chemically defined medium containing hepatocyte growth factor (HGF) and G5 supplement, was used to induce rhesus ...
2009 NOV 9 ... Medicine demonstrating that dendritic cells (DCs) scalably manufactured from human embryonicstem cells (hESCs) exhibit the normal functions of naturally occurring human DCs found ... (FDA) placed Geron's IND (Investigational New Drug application) for GRNOPC1, a cell therapy for neurologically complete, subacute spinal cord injury, on clinical hold ... matrices that support the growth and differentiation of hESCs in a defined (animal-free) medium. Conference Call At 8:00 a.m. PDT / 11:00 a.m. EDT on Friday, October 30, Thomas B. ...
2009 NOV 23 ... investigators in Milan, Italy report. "We deleted Sox2 in the mouse embryonic brain. At birth, the mice showed minor brain defects; shortly afterwards, however, NSCs ... "Neural stem cells (NSCs) are controlled by diffusible factors. The transcription factor Sox2 is ... published their study in Nature Neuroscience (Hippocampal development and neural stem cell maintenance require Sox2-dependent regulation of Shh. Nature Neuroscience, ... was partially rescued by the addition of SHH and was almost fully rescued by conditioned medium from normal cells," wrote R. Favaro and colleagues, University of Milan. ...
2009 NOV 16 ... Scientists in Belgium have successfully differentiated human embryonicstem cells (hESC) into major cell types of lung epithelial tissue using a convenient ... are important in the developing lung, to test whether their addition to the culture medium influences the differentiation pattern. Van Haute continues, "hESC have ...
2009 NOV 23 ... Research findings, 'Human embryonicstem cells secrete soluble factors that inhibit cancer cell growth,' are discussed in a ... molecular fractionation experiments were also performed. We found that hESC conditioned medium (hESC CM) inhibited SKOV-3 and HEY cell proliferation. Similar results were also ...
2009 OCT 19 ... hESCs, we determined (1) the temporal pattern of osteoblastic differentiation of human embryonicstemcell-derived mesenchymal stem cells (hESC-MSCs), (2) the influence of a ... cells derived from hESC-MSCs in calvarial defects. Incubation of hESC-MSCs in osteogenic medium induced osteoblastic differentiation of hESC-MSCs into mature osteoblasts in a similar ...
2009 NOV 16 ... Villejuif, France, "Embryoid bodies (EBs) generated during differentiation of human embryonicstem cells (hESCs) contain vascular-like structures, suggesting that commitment of ... Villejuif, Bone Morphogenetic Protein, Embryonic Stem Cells, Enzymes, Kinase, Stem Cell Research, INSERM. This article was prepared by Proteomics Weekly editors ... endothelial progenitors, we sorted them at day 7 and cultured them in endothelial medium. These cells were, however, unable to differentiate into endothelial cells. Under ...
2009 NOV 2 ... to test the embryotoxicity of benzene, toluene, xylene, and formaldehyde to murine embryonicstem cells using airborne exposure technique,' new data in stemcell research. ... determined using the MTT assay after further culture for 18 h at 37 degrees C in normal medium. The airborne IC(50) (50% inhibition concentration) of benzene, toluene, xylene, and ...
2009 OCT 12 ... we describe a novel approach to generating more mature insulin-producing cells from embryonicstem (ES) cells," scientists in the United States report. "A ... of mature pancreatic cells that function similarly to primary islets is the premise of cell therapy for diabetes. Here, we describe a novel approach to generating more mature ... showed a nearly fivefold (p < 0.05) increase when exposed to a high glucose (27.7 mM) medium. This 3D culture model provides an excellent system to study pancreatic endocrine ...
2009 OCT 12 ... serum-free, chemically defined conditions to generate fibrocartilage with human embryonicstem cells (hESCs). Three sequential experimental phases were performed to eliminate ... the understanding of mechanisms of chondrogenesis and creating clinically relevant stem cell therapies." Koay and colleagues published their study in Tissue ... self-assembly. Phase I studied serum effects, and showed that a 1% serum chondrogenic medium (CM) during differentiation resulted in uniform constructs, whereas a 20% serum CM did ...
2009 NOV 2 ... function." "BMP2- or transgene-induced Oct4 up-regulation drives human embryonic and induced pluripotent stem cells to become cardiac progenitors. When embryonicstem ... Evry, France, "Oct4 exerts a dose-dependent dual action, as both a gatekeeper for stem cell pluripotency and in driving cells toward specific lineages. Here, we identify the ... regulators of paracrine signals for cardiogenesis (i.e., Wnt, BMP2) released into the medium surrounding colonies of embryonic stem cells," wrote S. Stefanovic and colleagues, ...
2009 OCT 12 ... According to a study from the United States, "Embryonicstem cells (ESCs) with their abilities for extensive proliferation and multi-lineage ... Biomedical Engineering, Biomedicine, Biotechnology, Cytometry, Embryonic Stem Cell Research, Medical Device, Regenerative Medicine, Therapy, Tissue Engineering, Treatment, ... body cultures, all hESCs on microcarriers are exposed to soluble stimuli in the bulk medium facilitating efficient transition to DE. After reaching a peak concentration, hESCs in ...
2009 OCT 19 ... they may be replicable in vitro, with a goal of cellular replacement therapy. Human embryonicstemcell (hESC) lines were investigated as a model for parathyroid regeneration in ... parathyroid hormone [PTH]). We further characterized the cells by testing conditioned medium from various time points in our differentiation scheme for the presence of PTH. We found ...
2009 OCT 5 ... The Company is leveraging multiple stemcell technology platforms that include embryonicstem cells, induced pluripotent stem cells and tissue-derived (adult) stem cells. ... using its automated cell culture platform that enables large-scale production for medium-throughput screening of drug candidates. StemCells is also investing to ...
2009 NOV 1 ... conditions, supernatants from culture of bone fragments, marrow stroma cell line MS-5, embryonic fibroblast cell line NIH3T3, and a cocktail of epidermal growth factor (EGF) and ... According to a study from Naples, Italy, "The use of adult stem cells in tissue engineering approaches will benefit from the establishment of culture ... ability, and differentiation potency. In particular, using NIH3T3 conditioned medium, we obtained cells that showed impairment in osteogenic and chondrogenic differentiation ...
