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2009 NOV 1 ... (MC) carrying double-fusion reporter gene consisting of firefly luciferase and enhanced green fluorescent protein (Fluc-eGFP) for noninvasive measurement of transfection efficiency. Mouse C2C12 ...
2009 NOV 24 ... were severely reduced in number, and choroid plexuses were atrophic. This phenotype was enhanced when DeltaNp73-positive cells were ablated by diphtheria toxin expression. However, ... vivo, we generated a null allele and inserted sequences encoding the recombinase Cre and greenfluorescentprotein (EGFP). We show that DeltaNp73 is heavily expressed in the thalamic ...
2009 NOV 9 ... depletion mediated injury. To test the contribution of hydrogen peroxide, a subset of enhanced green fluorescent protein (EGFP) and SOD1 (serum free and injured) cells were treated with polyethylene ...
2009 NOV 3 ... (2) an increase in seed weight and fruit hexose level, which is likely achieved through enhanced sucrose hydrolysis in the apoplasm of the fruit vasculature. This assertion is based on ... putative invertase inhibitor, in tomato (Solanum lycopersicum). Expression of a INVINH1: greenfluorescentprotein fusion revealed its apoplasmic localization. Ectopic overexpression ...
2009 NOV 23 ... region, including both signal peptide (amino acids 1-26) and Pro(+2), was sufficient for enhanced green fluorescent protein to localize in the Golgi, whereas single amino acid mutation of Pro(+2) resulted in ...
2009 NOV 16 ... expressed by a Chinese hamster ovary cell line (CHO-TPO) and transiently expressed TPO-enhanced green fluorescent protein (eGFP) and -FLAG fusion proteins. Immunoblotting of CHO-TPO cell extracts showed ...
2009 NOV 1 ... type I and type II collagen promoters were linked to greenfluorescentprotein-topaz and enhanced cyan fluorescentprotein, respectively," investigators in the United States report. ...
2009 NOV 24 ... clustered GmNHX2 with organellar (tonoplast and vesicles) antiporters, the GmNHX2-EGFP (enhanced green fluorescent protein) fusion protein was possibly localized in the plasma membrane or organelle membrane of ...
2009 NOV 24 ... and independent of the presence of IcsA or functional FtsZ; native DnaK was found to be enhanced at midcell and the poles," wrote A. Janakiraman and colleagues, Massachusetts General ... cytoplasm, we screened a genome-scale library of Escherichia coli proteins tagged with greenfluorescentprotein (GFP) for those that displayed a localization pattern similar to ...
2009 NOV 16 ... the expression of HMGA1 in vivo, transgenic mice expressing endogenous; HMGA1 fused to enhanced green fluorescent protein were generated and then crossed with K19-Wnt1/C2mE mice, which develop gastric tumors ...
2009 NOV 3 ... persistence of gene expression." "In this study, we examined long-term enhancedgreenfluorescent-protein (eGFP) expression and vector genome persistence in the mouse ...
2009 NOV 16 ... the effect of Kit oncogenic mutations on its subcellular localization, we constructed enhanced green fluorescent protein (EGFP)-tagged human Kit chimeras harboring mutations either in the regulatory (V560G) or ...
2009 NOV 1 ... arcuate nucleus neurons. Therefore, we used ovariectomized oil-or E2-treated EGFP (enhanced green fluorescent protein)-GnRH mice to explore the expression and E2 regulation of T-type channels in GnRH ...
2009 NOV 16 ... protein-LC3, characteristic of autophagy. The combination ABT-737/rapamycin markedly enhanced sensitivity of H460 cells to radiation (dose enhancement ratio = 2.47; P = 0.002) in ... of apoptosis, and rapamycin correlated with an increase in punctate localization of greenfluorescent protein-LC3, characteristic of autophagy. The combination ABT-737/rapamycin ...
2009 NOV 3 ... "The conventional enhanced biological phosphorus removal (EBPR) system often deteriorates at low chemical oxygen ... syringae," scientists in Wuhan, People's Republic of China report. "A greenfluorescentprotein-fusion protein was expressed and used to confirm surface ...
2009 OCT 27 ... of all nine chemoreceptors in vivo using genome-encoded fusions to a variant of the enhanced green fluorescent protein and to monomeric red fluorescent protein. Six of the transmembrane (McpT to McpX and ...
2009 NOV 9 ... to express three different transgenes including the alkaline phosphatase (AP) gene, the enhanced green fluorescent protein (eGFP) gene, and a therapeutic microdystrophin gene (the Delta R4-23/Delta C). ...
2009 NOV 9 ... enhancer/promoter sequences of alpha 2(I) collagen gene (COL1A2) linked to enhanced green fluorescent protein (EGFP) or firefly luciferase (LUC) gene. Liver fibrosis was introduced into those mice ...
