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2009 NOV 27 ... remarkably high degree of identity (90%).""However, they display different expression patterns and show distinct kinetic properties, especially with regard to their ... All chimeric versions exhibited the same native structures as the parental proteins. Analysis of the chimeras constructed indicated that the region from amino acid residue 303 to the ...
2009 NOV 26 ... max (L.) Merrill] by particle bombardment and subsequent hygromycin selection. The expression of each enzyme was confirmed in leaves of the transgenic soybean plants by immunoblot ... of each enzyme was confirmed in leaves of the transgenic soybean plants by immunoblot analysis with specific antibodies and by measurement of PAT activity. The transgenic plants ...
2009 NOV 26 ... P-amylase activity, showed that an inactive allele of Amy beta gene (Amy beta-I) exists. expression analysis showed that Amy beta-I is transcribed normally, suggesting that translational control of ...
2009 NOV 27 ... mechanisms are not fully understood. We examined freeze-thaw injury by indirect gene expression analysis during postthaw incubation after freeze-thaw treatment using DNA microarray profiling. ...
2009 NOV 27 ... tumor cell line Bcap-37 is valid for bioactivity analysis of mammary gland specific expression vector."Zhang and colleagues published their study in Applied Biochemistry ...
2009 NOV 29 ... however, the results of RNA gel blot analysis indicated a strong correlation between the expression of the CiFT gene and floral bud differentiation ill vitro. Flowers that developed ill ...
2009 NOV 27 ... According to a study from Ebetsu, Japan, "This study is the first to examine the expression of the 14 monocarboxylate transporter genes (MCT1-MCT14) in the mammary gland of ... and MCT14 in cow mammary gland. The amplified cDNA segments were confirmed by sequence analysis and deposited in the GenBank. Using the commercially available antibodies against ...
2009 NOV 27 ... The genes identified here likely reflect the existence of distinct programs of gene expression governing the development, maturation, and function of the newly identified ... embryonic and postnatal development." "We used in situ hybridization analysis, immunocytochemistry, and retrograde labeling to define the layer-specific and ...
2009 NOV 27 ... is a powerful antioxidant. The current study demonstrates the effects of boldine on the expression of adiponectin and its regulators, CCAAT/enhancer binding protein-alpha (C/EBP Chi) and ... adipogenesis. Peroxisome proliferator response element-luciferase promoter transactivity analysis showed that boldine interacts with the PPAR response element and could potentially ...
2009 NOV 25 ... According to a study from Toulouse, France, "Plasmid Gene transfer and expression can be obtained by the application of electric pulses to a mixture of cells and plasmids ...
2009 NOV 26 ... define a type and effect system that, given a program, extracts a history expression, that is, a sound overapproximation to the set of histories obtainable at runtime. After ... "An extension of the lambda-calculus is proposed, to study resource usage analysis and verification. It features usage policies with a possibly nested, local scope, and ...
2009 NOV 27 ... system mediated by the tetracycline repressor (TetR) is widely used to study gene expression in prokaryotes and eukaryotes. To study multiple genes in parallel, a triple mutant ... in the absence and in complex with 4-ddma-atc at resolutions of 2.1 angstrom. Analysis of the structures in light of the available binding data and previously reported TetR ...
2009 NOV 26 ... with various concentrations of octanoate or decanoate. Cell viability, apoptosis, and expression of apoptosis-related proteins were investigated," researchers in the United States ... increased apoptosis, and increased reactive oxygen species production. Immunoblotting analysis showed an increase in the levels of cytoplasmic cytochrome c and cleaved poly(ADPribose) ...
2009 NOV 24 ... set of TIR-NBS-encoding genes in plants. However, the molecular mechanism underlying the expression of these genes is still unknown." "In this study, we presented a ... sequence decreased the translation efficiency of target gene by 88.3%. Additionally, the analysis of the transgenic P-985/UTR Delta/GUS plants showed that both the exon1 sequence and the ...
2009 NOV 24 ... site abrogated both JNK-mediated regulation as well as transcription factor binding. Expression of a dominant-negative SP-1 eliminated both the enhanced promoter activity and the ... binding site is necessary for TNF-alpha regulation. Electrophoretic mobility shift analysis demonstrated specific binding to this sequence, while site-directed mutagenesis of this ...
2009 NOV 24 ... has shown that, upon overexpression, TTC4 localizes to the cytoplasm. Interestingly, co-expression with a known protein interaction partner, hampin/MSL1, results in the nuclear ... chain reaction, we have observed that the murine TTC4 gene is ubiquitously expressed. Analysis of the TTC4 subcellular localization has shown that, upon overexpression, TTC4 localizes ...
2009 NOV 24 ... structural features that are highly conserved among the vertebrate orthologs. PepT1 mRNA expression can be detected in duodenum, jejunum and ileum from Tibetan pigs at 28 days by RT-PCR. ... in duodenum, jejunum and ileum from Tibetan pigs at 28 days by RT-PCR. Real-time PCR analysis indicated that the jejunum had the highest expression of PepT1 when compared with the ...
2009 NOV 24 ... all V harveyi isolates, some isolates did not cause hemolysis, indicating that vhh gene expression might be regulated. Analysis of the V harveyi HY01 genome revealed a V cholerae ...
2009 NOV 24 ... "We investigate the role of heterogeneous expression of IP3R and RyR in generating diverse elementary Ca2+ signals. It has been shown ... colleagues published their study in Bulletin of Mathematical Biology (A Computational Analysis of Localized Ca2+-Dynamics Generated by Heterogeneous Release Sites. Bulletin of ...
2009 NOV 24 ... scheme can account precisely for the dominant recovery rate of intact rods in which RGS9 expression varies over a 20-fold range. Furthermore, by screening the parameter space of the scheme ... researchers concluded: "All together, the new kinetic scheme and analysis explain how and why RGS9 concentration matters in rod phototransduction, and they ...
