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2009 JUN 22 ... for the isolation and propagation of neural progenitor and stem cells from human fetal tissue, with high rate of growth and high yields of differentiation into the neuronal, ... progenitor and stem cells were isolated from fetal forebrain tissue and propagated as neurospheres, in defined medium in the presence of leukemia inhibitory factor (LIF). Three protocols ...
2009 OCT 6 ... in Paris, France report. "Neurospheres containing NPs issued from fetal ganglionic eminences of transgenic mice expressing the green fluorescent protein (GFP) ...
2009 OCT 19 ... "Human fetal neural stem/progenitor cells (hNSCs) are investigated for their potential as a cell ... from different brain regions were long-term expanded in vitro to yield proliferating neurospheres giving rise to neurons, astro-, and oligodendroglial cells and assessed for their ...
2009 JAN 12 ... in 6 %-10 % cells. The results demonstrate for the first time that NSCs derived from the fetal hippocampus survive and give rise to all three CNS phenotypes following transplantation ... hippocampus of embryonic day 14 green fluorescent protein-expressing transgenic mice as neurospheres in vitro and grafted them into the hippocampus of 24-month-old F344 rats 4 days after ...
2009 JUN 22 ... that can give rise to all neural lineages. They are found in specific niches of fetal and adult brains and grow in vitro as non-adherent colonies, the neurospheres," ...
2009 APR 20 ... "Neurospheres can be generated from the mouse fetal forebrain by exposing multipotent neural stem cells (NSCs) to epidermal growth factor ...
2009 JAN 5 ... this study, the effects on survival, proliferation and neuronal differentiation of human fetal NSCs (hfNSCs) were observed after infection by a neurotrophin-3 (NT-3) recombinant ... (NT-3) recombinant retrovirus. The hfNSCs, from 12-week human fetal brains formed neurospheres, expressed the stem cell marker nestin and differentiated into the three main cell types ...
2009 AUG 24 ... in the SVZs bordering demyelinated white matter in multiple sclerosis brains. In human fetal brain, beta T4 cell densities peak during the latter stages of gliogenesis, which occurs ... SVZ of the lateral ventricles. beta T4 cells represent <2% of the cells present in neurospheres generated from postnatal rat brain but >95% of cells in neurospheres treated with the ...
2007 OCT 8 ... recovery method and correlated with pO2 measured in phantoms. pO2 images of human and fetal cerebrospinal fluid, bladder urine, and vitreous humor are presented and quantitative ... the hippocampus and the corpus callosum. Cells isolated from the SCZ and cultured as neurospheres behave as neural stem cells in vitro.
"Using electron and light ...
2007 AUG 13 ... progesterone treatment of PRL or PRL receptor (PRL-R) null mice is unable to prevent fetal loss. We have previously shown that the rat decidua is a site of PRL production and ... five magnetic resonance (MR) image-guided stereotactic intracerebral injections of HNSC neurospheres labeled with bromodeoxyuridine (BrdU) in the areas surrounding the ischemic lesion as ...
2008 APR 7 ... (DA) cell replacement therapy in Parkinson disease (PD) can be achieved using human fetal mesencephalic tissue; however, limited tissue availability has hindered further ... in the presence of key signals necessary for VM DA neuron development. Mouse VM neurospheres (VMNs) expanded with FGF2, differentiated with sonic hedgehog and FGF8, and transfected ...
2008 OCT 23 ... designed to establish an approach that allows direct comparisons between the biology of fetal and adult human PRPs, as a means to address potential differences in intrinsic ... properties of fetal and adult human PRPs. PDGF stimulated fetal human PRPs to generate neurospheres that differentiated primarily into oligodendrocytes, which acquired myelin basic protein ...
2007 AUG 27 ... New research, "Phenotypic characterization of neural stem cells from human fetal spinal cord: synergistic effect of LIF and BMP4 to generate astrocytes," is the subject ... Australia report.
"Fetal spinal cords were dissociated and expanded as neurospheres in medium with bone morphogenetic protein 4 (BMP4), leukemia inhibitory factor (LIF) or ...
2008 MAY 19 ... from the United States, "Human neural progenitor cells (hNPC) isolated from the fetal cortex can be expanded as aggregates of cells termed neurospheres. Traditional methods ...
2008 JUN 23 ... of dissociated neurosphere cells seen after differentiation triggered with adhesion and fetal calf serum by adding epidermal growth factor and basic fibroblast growth factor to the ... neurons and glial cells."
"Here we show that within 10 days of culture, neurospheres contained precursors and differentiated progeny of all three major central nervous ...
2008 MAR 17 ... "Ethanol (ETOH) consumption by pregnant women can result in Fetal Alcohol Spectrum Disorder (FASD). To date, the cellular targets and mechanisms ... in mitogenic media containing 0, 1, 10, or 100 mM ETOH. From 48 to 72 hours in culture, neurospheres generated in these conditions were filmed using time-lapse video microscopy. At the end ...
2007 JUL 30 ... in the journal Neurobiology of Disease, "Alcohol exposure during pregnancy may cause fetal alcohol syndrome (FAS), characterized by impaired cognitive functions. Neurogenesis ... caspase-3 12 h after ethanol treatment. Finally, we show that the number of primary neurospheres derived from the hippocampi of alcohol-exposed mice is reduced compared to controls. ...
