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2009 NOV 3 ... Investigators publish new data in the report 'RecA gene sequence and Multilocus Sequence Typing for species-level resolution of Burkholderia cepacia complex isolates.' ... and clinical origin, which failed to be identified by recA RFLP and species-specific PCR. By using recA sequence-based identification, 17 out of 26 BCC isolates were resolved at ...
2009 NOV 27 ... derived from wounds (108) and blood (99).""We performed spa typing and multilocus sequence typing (MLST) to determine clonal origin, and for selected strains ... of allergic diseases. The prevalence of 19 enterotoxin genes was determined by PCR in clinical S. aureus strains derived from wounds (108) and blood ...
2009 SEP 28 ... were determined by PCR and sequencing. Molecular epidemiology was analysed by PFGE and multilocus sequence typing. To elucidate possible mechanisms of fosfomycin resistance, uhpT, glpT, ...
2009 NOV 23 ... and its serovariants has been examined using multiple molecular techniques including multilocus sequence analysis, pulsed-field gel electrophoresis, and group-specific PCR analysis. ...
2009 NOV 1 ... electrophoresis) revealed that these E. coli O157 strains belong to four main clusters. Multilocus sequence typing analysis revealed that five housekeeping genes were identical in EHEC ... with similar frequencies. However, none of these virulence genes were detected by PCR in atypical O157 strains. Interestingly, five of nine atypical O157 strains produced ...
2009 OCT 20 ... no association between single SNPs and spine or hip bone mineral density (BMD). In the multilocus analysis, some haplotypes showed a slight association with spine BMD (P = 0.03), but it ... of WNT10B and other Wnt ligands were detected in human bone samples by real-time PCR. However, there was no relationship between genotype and RNA abundance," wrote J.L. ...
2009 OCT 19 ... isolates were characterised via toxin gene profiling, SCCmec, spa and agr typing, multilocus sequence typing (MLST) and minimum inhibitory concentration (MIC) determinations. ... patients located across U.K. and Wales were identified by polymerase chain reaction (PCR)," researchers in London, the United Kingdom report. "Representative ...
2009 OCT 19 ... leishmaniasis (VL). PCR-sequencing of the ribosomal internal transcribed spacer 1 and multilocus microsatellite typing (MLMT) were applied to DNA extracted from preparations of ...
2009 OCT 12 ... study in Diagnostic Molecular Pathology (dcDegenerate Oligonucleotide Primed-PCR for Multilocus, Genome-wide Analysis From Limited Quantities of DNA. Diagnostic Molecular Pathology, ...
2009 OCT 6 ... were analyzed through ABI 3100 Genetic Analyzer. Pentaplex PCR system was developed for multilocus amplification at the same time. For each locus 4-9 alleles were noted. Altogether, 32 ...
2009 OCT 12 ... "Here, we examined genetic variation, selection, and recombination in seven multilocus sequence typing (MLST) loci from 94 invasive, colonizing, and bovine strains ... and bovine strains representing 38 GBS sequence types and performed DNA sequencing and PCR-based restriction fragment length polymorphism analysis of several putative virulence ...
2009 NOV 10 ... sequences predicted divergent evolutionary histories distinct from those predicted by a multilocus sequence typing phylogeny based on partial sequencing of seven housekeeping genes," ... Agriculture. The researchers concluded: "Real-time reverse transcription-PCR indicated that both genes are expressed in all of the strains." Mullins ...
2009 SEP 1 ... of strain 1289 compared to that of RB50 was partially attributable to the TTSS. Using multilocus sequence typing, we identified another strain from the same lineage as strain 1289. ... host, strain RB50. Transcriptome analysis and quantitative reverse transcription-PCR showed that the type III secretion system (TTSS) genes were more highly expressed by ...
2009 AUG 24 ... "Genetic relatedness was determined by pulsed-field electrophoresis with XbaI and multilocus sequence typing. A total of 67 patients with incident bloodstream infections were ... geographical region. Molecular characterization was done by using isoelectric focusing; PCR; and sequencing of the bla(CTX-M-), bla(TEM-), bla(OXA-), bla(SHV-), and ...
2009 NOV 2 ... Guerdern and colleagues published their study in Parasitology (Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in ... The researchers concluded: "Furthermore, using a novel species-specific PCR based on the TPI gene, mixed infections with both assemblage A and B were detected in a ...
2009 SEP 14 ... France report. "Phage genetic diversity was evaluated by a PCR-based multilocus typing method targeting key modules located in the packaging, structural, host lysis, ...
2009 AUG 24 ... penicillin-resistant pneumococci were typed by PFGE and selected strains were studied by multilocus sequence typing (MLST). The presence of genes related to the Tn916 family of transposons ... The presence of genes related to the Tn916 family of transposons was investigated by PCR. From a total of 1197 invasive pneumococcal isolates of serotype 19A received at the ...
