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2009 NOV 28 ... was successfully detected in a sample of potato stew by conventional RT-seminested-PCR," wrote C. Mayr and colleagues.The researchers concluded: "Sequence ... Mayr and colleagues.The researchers concluded: "Sequence comparison of the amplification products obtained from the potato stew and a stool sample from an infected child ...
2009 NOV 23 ... management is applied. To determine the usefulness of semi-quantitative differential PCR (SQ-PCR) for accurate quantification of MYCN gene copy number, we evaluated the ... recent research published in the Brazilian Journal of Medical and Biological Research, "Amplification of the MYCN gene in neuroblastomas is a potent biological marker of highly aggressive ...
2009 NOV 23 ... sequencing of the kinase domain of EGFR (exons 18-21) was performed using nested PCR amplification of individual exons. Forty-eight patients (41.8%) harbored exon 19 deletion or exon 21 ...
2009 NOV 23 ... with the data from the gene expression microarray and were validated by quantitative PCR. Clinical association of the defined target genes was analyzed by fluorescence in situ ... were validated by quantitative PCR. Fluorescence in situ hybridization studies revealed amplification of GPR160 (in 25% of cases) and SKIL (33%); and deletion of ADAMTS9 (30%), LRIG1 (35%), ...
2009 NOV 26 ... origin. Furthermore, we could amplify these 2 STS markers' fragments by using multiplex PCR reproducibly," wrote Y. Okuda and colleagues, Tottori University.The ... SD296, and their implementations in various genetic backgrounds were validated by the amplification test against 15 wild type isolates from different geographical origin. Furthermore, we ...
2009 NOV 27 ... of bacteria. The goal of this study was the development of a Cryptosporidium PMA-PCR (CryptoPMA-PCR) assay that includes PMA treatment prior to PCR analysis in order to ... assay that includes PMA treatment prior to PCR analysis in order to prevent the amplification of DNA from dead oocysts. The results demonstrated that PMA penetrates only dead oocysts ...
2009 NOV 24 ... for alpha-thalassaemia with the --(SEA) genotype. A PGD protocol using fluorescent gap PCR was performed for 51 cycles on 43 couples with the --(SEA) genotype." ... 51 cycles on 43 couples with the --(SEA) genotype." "Allele drop-out and amplification failure rates were retrospectively analyzed. A total of 472 embryos were biopsied. ...
2009 NOV 23 ... the CTG repeat region of the DMPK gene based on a two-step polymerase chain reaction (PCR) protocol. (1) For the detection of alleles of up to 100 repeats, a quantitative ... (1) For the detection of alleles of up to 100 repeats, a quantitative fluorescent (QF) amplification with primers flanking the repeat region of the DM1 locus and two reference genes (PAX2 ...
2009 NOV 24 ... report. "Lymph nodes were screened for the presence of any bacteria by PCR amplification and sequencing targeting the 16 S rRNA gene and also by a specific real-time PCR ...
2009 NOV 23 ... enabling on-chip assays of multiple biomarkers, and (ii) able to perform direct (PCR-free) readout of disease-related genes. Here we report a chip onto which we integrate ... nanostructured microelectrode integrated circuit reported herein provides direct, amplification-free, sample-to-answer in under 1 h using the 10 ng of mRNA readily available in biopsy ...
2009 NOV 23 ... (5 accessions). Genomic DNA regions of As120a, avenin, and globulin were amplified by PCR," scientists in Osaka, Japan report. "A total of 130 polymorphic ... fragments with 11 restriction enzymes. The number of fragments generated by PCR-amplification followed by digestion with restriction enzymes was almost the same as those among the ...
2009 NOV 23 ... (p <0.002), but this lower yield had little effect on polymerase chain reaction (PCR) amplification," wrote M.F. Baay and colleagues, University of Antwerp. ...
2009 NOV 23 ... amplification. All of the 105 isolates from Omugo that we successfully analysed by PCR-RFLP possessed the TbAT1 wild type allele. In addition, PCR/RFLP analysis was performed ...
2009 NOV 27 ... in the xanthomonads, and the sequence was used to design a specific PCR primer set. The PCR amplification using the primer set amplified a 777-bp specific DNA fragment for all xanthomonad ...
2009 NOV 24 ... In total, 496 symptomatic patients were included. Five polymerase chain reaction (PCR) assays and one real-time nucleic acid sequence based amplification (NASBA) assay, ...
2009 NOV 26 ... "Allergen detection methods for peanut, soybean, and wheat were developed by designing PCR primer pairs for specific amplification of a fragment of the internal transcribed spacer ...
2009 NOV 24 ... a region of the small subunit of the nuclear ribosomal RNA (SSU rRNA) gene using nested PCR and Subsequent sequencing. The sequences front 15 isolates from G. duodenalis were ... Lumpur, Malaysia report. "Molecular identification was carried Out by the amplification of a region of the small subunit of the nuclear ribosomal RNA (SSU rRNA) gene using ...
2009 NOV 24 ... host and parasite sequences were identified, of which 20 encoded ribosomal proteins. PCR for seven predicted parasite and two host genes were used to confirm attributions. The ... sequence variants, but parasite rps27a contained only one polymorphism, likely due to an amplification error. Amplification, cloning, and sequencing of five parasite protein-coding genes ...
2009 NOV 23 ... The TrovaGene HPV DNA test involves isolation of DNA from urine and specific PCR amplification of the HPV E1 region to detect the presence of high-risk HPV types. In a recent study ...
