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2009 NOV 24 ... higher than that of the conventional gel-based PCR assay targeting the same gene. The real-time PCR was reproducible, as shown by satisfactory low intraassay and interassay coefficients of ...
2009 NOV 25 ... quantitative identification of each clone within corresponding fraction using real time PCR analysis. Stable clones differed in phenotype and several were shown to belong to the ...
2009 NOV 27 ... during incubation of peat slurries at temperatures from 4 to 37 degrees C, and real-time quantitative PCR was applied to quantify the abundances of mcrA genes and transcripts; ...
2009 NOV 24 ... The researchers concluded: "Validation of a number of genes by quantitative real-time PCR indicated that P4 supplementation advances endometrial gene expression by altering the ...
2009 NOV 24 ... Primers for these three genes were designated according to known partial sequences. PCR products amplified from total DNA were labeled with digoxigenin (DIG) by random priming ...
2009 NOV 24 ... Fresh data on biopsy are presented in the report 'Real-time PCR strategy and detection of bacterial agents of lymphadenitis.' "The aim of this study ...
2009 NOV 26 ... was growing. Leaves from lettuce plants and seedlings were examined for viruses by real-time RT-PCR. When the lettuce plants were exposed to very high concentrations of CaCV, the ...
2009 NOV 24 ... wrote A. Rebl and colleagues. The researchers concluded: "Quantitative Real-Time RT-PCR revealed that delta-COP is differentially expressed in liver and gill tissue of ...
2009 NOV 24 ... chain and signal transduction genes etc. were selected for real time quantitative RT-PCR analyses, and their possible functions related to the molecular mechanism of ...
2009 NOV 26 ... illness. The purpose of this study was to compare standard culture-based methods and real-time polymerase chain reaction (RT-PCR) for detection of Campylobacter jejuni from retail ...
2009 NOV 24 ... using flow cytometry, and chemo me mRNA levels in the skin were quantified by means of real-time PCR. The total number of CD1a(+) epidermal DCs increased and the proportion of ...
2009 NOV 27 ... tend to yield poor quality DNA in low copy number. The first approach adopted a real-time PCR method in which primers were used to amplify a small fragment in the mitochondrial ...
2009 NOV 24 ... of five genes (TLR2, IRAK2, IL1R2, C20orf114, and SERPINB3) was confirmed using real-time polymerase chain reaction (PCR). These observations suggest that depletion of dietary ...
2009 NOV 24 ... symptomatic patients were included. Five polymerase chain reaction (PCR) assays and one real-time nucleic acid sequence based amplification (NASBA) assay, developed by three Russian ...
2009 NOV 26 ... the TLRs. was evaluated by semi-quantitative PCR on an epithelial/bacteria co-culture. Real-time PCR was used to monitor expression of TLRs and cytokines in a monocytic cell line (THP-1) ...
2009 NOV 24 ... can be detected in duodenum, jejunum and ileum from Tibetan pigs at 28 days by RT-PCR. Real-time PCR analysis indicated that the jejunum had the highest expression of PepT1 when compared ...
2009 NOV 23 ... were significantly higher at 12 72 hours post injection (P <0.05). Quantitative real-time PCR indicated that Pm-OST1 was comparably expressed throughout ovarian development in normal ...
2009 NOV 24 ... by virus isolation in cell culture. The subtype of recovered strains was determined by real-time PCR. Of 368 RSV infections identified in children during this interval, subtype A virus ...
2009 NOV 27 ... rate for all-trans-retinoic acid, followed by beta-apo-13-carotenone (C-18). As shown by real-time RT-PCR, CYP120A1 is expressed under normal growth conditions and is slightly induced by ...
2009 NOV 23 ... and enzymes onto content-ready SmartChips for use with WaferGen's SmartChip™ Real-Time PCR System. The SmartChip Real-Time PCR System is designed ...
