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2009 NOV 17 ... by acidic, polyacrylamide gel electrophoresis (A-PAGE), and two-dimensional A-PAGE x SDS-PAGE. All accessions were confirmed to contain equal amounts of two polypeptide chains ...
2009 NOV 23 ... (ii) surface plasmon resonance spectroscopy, and (iii) two-dimensional blue native/SDS-PAGE. Recombinant protein as well as AMPK in cell lysates of primary cells revealed ...
2009 NOV 10 ... was electro-transformed into the B. thuringiensis subsp. kurstaki Cry(-)B. Analyzed by SDS-PAGE and Western blotting, the transformant Cry(-)B-pHAc-CDEP2 strain produced a 130 kDa ...
2009 NOV 23 ... E. coli is a homodimer, whereas the native aArDH in A. suboxydans is a homotetramer. On SDS-PAGE, the recombinant and native aArDH give one protein band at the position ...
2009 NOV 9 ... process may include ELISA and indirect ELISA, Western blot, antibody isotype, SDS-PAGE, surface plasma resonance, immunohistochemistry, immunoprecipitation, ...
2009 NOV 26 ... the molecular weight for the partially purified peptide (3,420 Da) was carried out using SDS-PAGE stained by silver stain and with a bioindicator (L. monocytogenes). The purified ...
2009 NOV 24 ... "Homogeneous beta-xylosidases with molecular mass values 120 and 80 kDa (as shown by SDS-PAGE), belonging to the third family of glycosyl hydrolases, were isolated by ...
2009 NOV 9 ... "Mass spectroscopic analysis was performed on protein bands obtained after 1-D SDS-PAGE of co-precipitation eluates from cell extracts of mitomycin C treated and untreated ...
2009 NOV 23 ... or inhibited human platelets. Co-purified plek-binding proteins were resolved by SDS-PAGE and identified via nanospray quadruple TOF MS. Identified proteins may be involved ...
2009 NOV 9 ... a plasmid-mediated AmpC beta-lactamase, DHA-1, in addition to TEM-1 and CTX-M-14. SDS-PAGE analysis of the outer-membrane proteins (OMPs) revealed that both isolates lacked ...
2009 NOV 10 ... showed a single major band with a relative molecular mass of approximately 25,000 on SDS-PAGE. A cDNA encoding glyoxalase I was cloned and sequenced. Sequence comparison ...
2009 NOV 3 ... a significant increase of the total protein concentration in the perfusate. Furthermore, SDS-PAGE analysis of the perfusate revealed three new proteins with molecular masses of 16, ...
2009 NOV 24 ... and to compare them with self prepared extracts of different cattle breeds by means of SDS-PAGE and immunoblotting using the sera of 42 German farmers with asthma and ...
2009 NOV 23 ... solubly expressed; no soluble GSIMH and HGSIMH were observed, based on the result of SDS-PAGE." "Gel filtration of purified soluble HGSIM showed that hexamers and ...
2009 NOV 23 ... (HPLC-MS/MS); second, protein separation by denaturing one-dimensional electrophoresis (SDS-PAGE) followed by HPLC-MS/MS analysis. Finally, two-dimensional polyacrylamide gel ...
2009 NOV 9 ... of Phragmites was established based on the swamp ecotype. Proteins were resolved by 2-D/SDS-PAGE and identified by MALDI-TOF/TOF MS. In total, 177 spots were identified ...
2009 NOV 9 ... weight of the highly purified protein, designated MML, was 40 kDa as determined by SDS-PAGE analysis. MML exhibited significant cytotoxicity to several cancer cell types, ...
2009 NOV 2 ... with type 2 diabetes. In vitro glycated TAR showed an altered migration pattern on SDS-PAGE due to aggregation. Glycated TAFI showed decreased activity after activation by ...
