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2009 NOV 23 ... "The Ver2 protein was expressed in bacteria as a recombinant GST-Ver2 fusion protein. The purified recombinant protein was further characterized using an affinity ...
2009 NOV 23 ... is the accepted animal model for RSV infection. MDT-637 acts by targeting the viral fusion protein, which is the only validated target for RSV treatments. The compound is inhaled, which ...
2009 NOV 27 ... According to recent research from the United States, "Membrane fusion without lysis has been reconstituted with purified yeast vacuolar SNAREs (soluble ... purified yeast vacuolar SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), the SNARE chaperones Sec17p/Sec18p and the multifunctional HOPS complex, ...
2009 NOV 23 ... to the green fluorescent protein (GFP). The analysis of the subcellular localization of fusion proteins revealed that an internal targeting signal of TcPGM, residing between amino ...
2009 NOV 23 ... "The plasmid was transformed into strains of S. gordonii expressing the fusion protein SpaP/S1, the anti-complement receptor 1 (CR1) single-chain variable fragment (scFv) ...
2009 NOV 23 ... we demonstrated that Pro(+2) was important for the enhanced green fluorescent protein fusion protein to concentrate at a transport vesicle formation site within the ER, often termed the ER ...
2009 NOV 23 ... for non-invasive dual-modality imaging of ovarian cancer virotherapy. A functional fusion protein composed of fluorescent and nuclear imaging tags was genetically incorporated into the ...
2009 NOV 23 ... People's Republic of China report. "Rabbits were nasally immunized with a fusion protein HSP65-6 x P277 10 times every other day. Immunologic results, including the repressed ...
2009 NOV 24 ... and vesicles) antiporters, the GmNHX2-EGFP (enhanced green fluorescent protein) fusion protein was possibly localized in the plasma membrane or organelle membrane of transgenic plant ...
2009 NOV 23 ... way to add new reagents to pre-formed droplets is by controlled, pairwise droplet fusion. We describe a droplet-based microfluidic system capable of performing multiple ... reagents, the end-point and kinetic analysis of the catalytic activity of the translated protein. In vitro translation and the laccase assay must be performed sequentially as the ...
2009 NOV 24 ... chaperone DnaK both mimicked IcsA localization at elevated temperatures as a GFP fusion and was required for the localization of IcsA to the pole in the cytoplasm of E. coli. ... localizes to poles and potential cell division sites independent of the cell division protein FtsZ. To identify bacterial proteins involved in the targeting of IcsA to the pole in ...
2009 NOV 24 ... under LD conditions," scientists in Takayama, Japan report. "An RFT1: GFP fusion protein localized in the shoot apical meristem (SAM) under LD conditions, suggesting that RFT1 ...
2009 NOV 23 ... leukemia is defined by the acquired genetic mutation, t(9;22), which leads to the fusion-protein BCR-ABL. Prior to the development of imatinib mesylate (Gleevec), treatment was ...
2009 NOV 23 ... revealed that a 131 amino acid N-terminal fragment of Ath1 was sufficient to target the fusion protein to the cell surface, enabling growth of the suc2D mutant on sucrose. The unique ...
2009 NOV 27 ... binding of maspin to alpha 5 beta 1 was confirmed using a recombinant alpha 5 beta 1-Fc fusion protein. Using conformation-dependent anti-beta 1 antibodies, maspin binding to VSMC was found ...
2009 NOV 23 ... encephalitis virus (JEV) interacts with a cellular receptor and mediates membrane fusion to allow viral entry into target cells, thus eliciting neutralizing antibody response. ... "As noted in other flaviviruses, the envelope (E) protein of Japanese encephalitis virus (JEV) interacts with a cellular receptor and mediates ...
2009 NOV 23 ... of a novel bacterial AB(5) toxin." "Here, we report that an engineered fusion protein, epidermal growth factor (EGF)-SubA, combining EGF and SubA, is highly toxic to growing ...
2009 NOV 28 ... up and is exposed to the antibodies for just a few minutes during the process of cell fusion and infection.But the problem for infection control is that such powerful ... of the virus - the so-called outer coat membrane proximal region - a part of the outer protein coating next to the viral membrane that opens up and is exposed to the antibodies for ...
2009 NOV 23 ... G (PknG) in Mycobacterium tuberculosis has been shown to modulate phagosome-lysosome fusion. The protein has three distinct domains, an N-terminal Trx domain, a kinase domain, and ...
2009 NOV 23 ... unclear." "In this article, we investigate beta-cell mitochondrial fusion and fission in detail and report alterations in response to various combinations of ... combinations of nutrients. Using matrix-targeted photoactivatable green fluorescent protein, mitochondria were tagged and tracked in beta-cells within intact islets, as isolated ...
